A simplified method for CRISPR-Cas9 engineering of Bacillus subtilis
A. J. Sachla, A. J. Alfonso, J. D. Helmann
This paper introduces a simplified CRISPR-Cas9 method for editing Bacillus subtilis by targeting an existing erythromycin resistance cassette, eliminating the need for custom gRNA design and cloning.
bioRxiv · Jun 29, 2021Microbiology