J. D. Helmann

The Bacillus subtilis yqgC-sodA operon protects magnesium-dependent enzymes by supporting manganese efflux

This paper shows that deletion of the yqgC-sodA operon in Bacillus subtilis causes Mn sensitivity by reducing Mn efflux and inhibiting Mg-dependent enzymes, distinct from efflux-deficient mutants.

The Bacillus subtilis cell envelope stress-inducible ytpAB operon modulates membrane properties and contributes to bacitracin resistance

This paper shows that the ytpAB operon, induced by cell wall inhibitors, helps Bacillus subtilis resist bacitracin by modulating membrane properties and interacting with resistance proteins.

TerC Proteins Function During Protein Secretion to Metalate Exoenzymes

The paper shows that TerC family proteins are involved in metalation of enzymes during secretion via the Sec pathway, specifically for manganese-dependent enzymes.

A decrease in fatty acid synthesis rescues cells with limited peptidoglycan synthesis capacity

The paper shows that reducing fatty acid synthesis rescues bacteria with limited peptidoglycan synthesis capacity by balancing membrane and cell wall synthesis.

Mutations in rpoB that confer rifampicin resistance can alter levels of peptidoglycan precursors and affect β-lactam susceptibility

This paper studies how rpoB mutations in Bacillus subtilis confer rifampicin resistance and affect β-lactam susceptibility through changes in peptidoglycan precursor levels.

A simplified method for CRISPR-Cas9 engineering of Bacillus subtilis

This paper introduces a simplified CRISPR-Cas9 method for editing Bacillus subtilis by targeting an existing erythromycin resistance cassette, eliminating the need for custom gRNA design and cloning.

Mutations of the YidC Insertase alleviate stress from σM-dependent membrane protein overproduction in Bacillus subtilis

Activation of a zinc metallochaperone by the alarmone ZTP

This study identifies ZagA, a zinc metallochaperone in Bacillus subtilis, which interacts with FolE to sustain folate synthesis under zinc limitation, activated by the alarmone ZTP.

Genome-wide characterization of the Fur regulatory network reveals a link between catechol degradation and bacillibactin metabolism in Bacillus subtilis

This paper identifies Fur binding sites in Bacillus subtilis and links catechol degradation to bacillibactin metabolism, showing CatDE detoxifies endogenous catechol derivatives.