Structural and Dynamic Impacts of Single-atom Disruptions to Guide RNA Interactions within the Recognition Lobe of Geobacillus stearothermophilus Cas9
H. B. Belato, A. L. Knight, A. M. D'Ordine, C. Pindi, Z. Fan, J. Luo, G. Palermo, G. Jogl, G. P. Lisi
This study investigates how single-atom mutations in the recognition lobe of GeoCas9 affect its structure, dynamics, and guide RNA interactions without altering its functional specificity.
eLife · May 19, 2025Peer-reviewedBiophysics