Human Cells for Human Proteins: Isotope Labeling in Mammalian Cells for Functional NMR Studies of Disease-Relevant Proteins
P. Rössler, M. Ruckstuhl, A. Löbbert, T. Stühlinger, L. R. Franchini, C.-J. Tsai, R. Lichtenecker, B. Shrestha, S. H. Rüdisser, R. Konrat, G. F. X. Schertler, A. D. Gossert
The paper develops cost-effective isotope labeling protocols in HEK293 cells for NMR studies of difficult-to-produce human proteins, enabling detailed structural and functional analysis.
bioRxiv · Apr 9, 2024Biophysics