Plant BiologybioRxiv
A normalization method that controls for total RNA abundance affects the identification of differentially expressed genes, revealing bias toward morning-expressed responses
K. Laosuntisuk, A. R. Vennapusa, I. M. Somayanda…·C. J. Doherty
The paper evaluates how normalization methods affect differential gene expression analysis in sorghum, showing that spike-in controls reveal biases in gene expression related to time of day and chilling stress.
DOI 10.1111/tpj.16654