A normalization method that controls for total RNA abundance affects the identification of differentially expressed genes, revealing bias toward morning-expressed responses
K. Laosuntisuk, A. R. Vennapusa, I. M. Somayanda, A. R. Leman, S. K. Jagadish, C. J. Doherty
The paper evaluates how normalization methods affect differential gene expression analysis in sorghum, showing that spike-in controls reveal biases in gene expression related to time of day and chilling stress.
bioRxiv · Oct 28, 2023Plant Biology