Detection and discrimination of single nucleotide polymorphisms by quantification of CRISPR-Cas catalytic efficiency
C. Blanluet, D. A. Huyke, A. Ramachandran, A. S. Avaro, J. G. Santiago
This paper quantifies CRISPR-Cas12 catalytic efficiency to detect and discriminate SNPs, finding that kinetic parameters like KM and [Formula] offer better discrimination than endpoint fluorescence.
bioRxiv · Apr 22, 2022Bioengineering