J. G. Santiago

This paper quantifies CRISPR-Cas12 catalytic efficiency to detect and discriminate SNPs, finding that kinetic parameters like KM and [Formula] offer better discrimination than endpoint fluorescence.

This study measures kinetic parameters and limits of detection for CRISPR-Cas12 and Cas13 enzymes in amplification-free diagnostics, revealing typical catalytic efficiencies and picomolar detection limits.

The paper models CRISPR enzyme kinetics using Michaelis-Menten theory to assess detection limits and assay times for Cas12 and Cas13 enzymes in diagnostics.

This paper develops an electric-field-driven microfluidic system for CRISPR-based diagnostics, achieving SARS-CoV-2 detection in 30 minutes from raw sample to result.