Author

D. A. Huyke

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 3

Signature Assays, Organisms & Methods

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Approach:statistical model· 2 Research Focus:benchmarking· 1 Assay:CRISPR screen· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Assay:proteomics· 1 Research Focus:variant effect prediction· 1

Frequent Co-Authors

A. Ramachandran· 3 shared J. G. Santiago· 3 shared A. S. Avaro· 1 shared B. A. Pinsky· 1 shared C. Blanluet· 1 shared E. K. Kotseroglou· 1 shared E. Sharma· 1 shared M. K. Sahoo· 1 shared

Activity by Year

2020-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBioengineering

A. Ramachandran

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3 papers

Recent·Most cited

Detection and discrimination of single nucleotide polymorphisms by quantification of CRISPR-Cas catalytic efficiency

C. Blanluet, D. A. Huyke, A. Ramachandran, A. S. Avaro, J. G. Santiago

This paper quantifies CRISPR-Cas12 catalytic efficiency to detect and discriminate SNPs, finding that kinetic parameters like KM and [Formula] offer better discrimination than endpoint fluorescence.

bioRxiv · Apr 22, 2022Bioengineering

Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics

D. A. Huyke, A. Ramachandran, V. I. Bashkirov, E. K. Kotseroglou, T. Kotseroglou, J. G. Santiago

This study measures kinetic parameters and limits of detection for CRISPR-Cas12 and Cas13 enzymes in amplification-free diagnostics, revealing typical catalytic efficiencies and picomolar detection limits.

bioRxiv · Jan 31, 2022 · PreprintBioengineering

Electric-field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2

A. Ramachandran, D. A. Huyke, E. Sharma, M. K. Sahoo, N. Banaei, B. A. Pinsky, J. G. Santiago

This paper develops an electric-field-driven microfluidic system for CRISPR-based diagnostics, achieving SARS-CoV-2 detection in 30 minutes from raw sample to result.

bioRxiv · May 21, 2020Bioengineering