A. Ramachandran

PATHOGEN GROWTH AND VIRULENCE DYNAMICS DRIVE THE HOST EVOLUTION AGAINST COINFECTIONS

This study shows that host evolution against coinfections is influenced by the growth and virulence dynamics of individual pathogens, with fast-acting pathogens hindering adaptive success.

Caenorhabditis elegans model for in vivo screening of human gut microbiota mediated colonization and colonization resistance against Clostridioides difficile

This study uses C. elegans to screen human gut microbiota for colonization and resistance against C. difficile, finding 46% colonization success and 50% in vivo inhibition of C. difficile.

Free-floating Bacteria Transcriptionally Respond to Shear Flow

This study shows free-floating bacteria detect shear flow to alter gene expression, enhancing survival in low oxygen conditions through quorum sensing and oxygen gradient formation.

Systemic 4-1BB stimulation augments extrafollicular memory B cell formation and recall responses during Plasmodium infection.

This study shows that 4-1BB stimulation enhances memory B cell formation and recall responses during Plasmodium infection, improving protection against reinfection.

Klebsiella pneumoniae Co-infection Leads to Fatal Pneumonia in SARS-CoV-2-infected Mice

This study shows that co-infection with SARS-CoV-2 and Klebsiella pneumoniae in mice leads to severe pneumonia and higher mortality.

Loss of FOXP3 function causes expansion of two pools of autoreactive T cells in patients with IPEX syndrome.

FOXP3 mutations in humans lead to expansion of autoreactive T cells from both effector and regulatory T cells that gain effector function.

Detection and discrimination of single nucleotide polymorphisms by quantification of CRISPR-Cas catalytic efficiency

This paper quantifies CRISPR-Cas12 catalytic efficiency to detect and discriminate SNPs, finding that kinetic parameters like KM and [Formula] offer better discrimination than endpoint fluorescence.

Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics

This study measures kinetic parameters and limits of detection for CRISPR-Cas12 and Cas13 enzymes in amplification-free diagnostics, revealing typical catalytic efficiencies and picomolar detection limits.

Enzyme kinetics of CRISPR molecular diagnostics

The paper models CRISPR enzyme kinetics using Michaelis-Menten theory to assess detection limits and assay times for Cas12 and Cas13 enzymes in diagnostics.

A metagenomic-based stufy of two sites from the Barbadian reef system

The paper analyzes the microbiome of two Barbadian coral reef sites using metagenomic sequencing to compare microbial community composition in relation to environmental factors.

Electric-field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2

This paper develops an electric-field-driven microfluidic system for CRISPR-based diagnostics, achieving SARS-CoV-2 detection in 30 minutes from raw sample to result.

Diversity and biogeography of SAR11 bacteria from the Arctic Ocean

The paper investigates SAR11 bacterial diversity in the Arctic Ocean, identifying distinct ecotypes and their association with water masses and depth.

Mitochondrial RNA capping: highly efficient 5’-RNA capping with NAD+ and NADH by yeast and human mitochondrial RNA polymerase

The paper shows mitochondrial RNA polymerases in yeast and human cells efficiently cap RNA with NAD+ and NADH, linking metabolism to mitochondrial gene expression.

Microbial life under ice: metagenome diversity and in situ activity of Verrucomicrobia in seasonally ice-covered lakes

This study examines Verrucomicrobia diversity and metabolic activity under ice in northern lakes using metagenomics and metatranscriptomics, revealing their active role in ice-covered environments.

Whole genome sequencing-based detection of antimicrobial resistance and virulence in non-typhoidal Salmonella enterica isolated from wildlife

This study sequenced whole genomes of non-typhoidal Salmonella enterica isolates from wildlife to identify antimicrobial resistance and virulence genes.