Thermodynamic coupling between neighboring binding sites in homo-oligomeric ligand sensing proteins from mass resolved ligand dependent population distributions
W. Li, A. S. Norris, K. Lichtenthal, S. Kelly, E. C. Ihms, P. Gollnick, V. H. Wysocki, M. P. Foster
The paper quantifies thermodynamic coupling between ligand binding sites in homo-oligomeric proteins using statistical models and native mass spectrometry, revealing large differences in cooperativity.
bioRxiv · Mar 19, 2022Biophysics