K. Lichtenthal

Structural basis of nearest-neighbor cooperativity in the ring-shaped gene regulatory protein TRAP from protein engineering and cryo-EM

This study investigates nearest-neighbor cooperativity in TRAP by engineering dTRAP variants and using cryo-EM and thermodynamic analyses to reveal structural and thermodynamic mechanisms of ligand binding cooperativity.

Thermodynamic coupling between neighboring binding sites in homo-oligomeric ligand sensing proteins from mass resolved ligand dependent population distributions

The paper quantifies thermodynamic coupling between ligand binding sites in homo-oligomeric proteins using statistical models and native mass spectrometry, revealing large differences in cooperativity.