V. H. Wysocki

Time resolved multi-omics reveals diverse metabolic strategies of Salmonella during diet-induced inflammation

This study uses multi-omics to analyze Salmonella's metabolic strategies in mice on different diets over time, identifying phase-specific gene expression linked to infection stages and therapeutic targets.

Stepwise design of pseudosymmetric protein hetero-oligomers

The paper introduces a method to design pseudosymmetric protein hetero-oligomers by redesigning symmetric homo-oligomers and recombining them to form new hetero-oligomers with high structural specificity.

Protein Quaternary Structures in Solution are a Mixture of Multiple forms

The paper assesses the oligomeric states of 17 bacterial proteins using multiple techniques, finding that most exist in multiple forms and that AlphaFold Multimer better predicts their quaternary structures than existing databases.

Thermodynamic coupling between neighboring binding sites in homo-oligomeric ligand sensing proteins from mass resolved ligand dependent population distributions

The paper quantifies thermodynamic coupling between ligand binding sites in homo-oligomeric proteins using statistical models and native mass spectrometry, revealing large differences in cooperativity.

Structure of a Rad52 homolog from bacteriophage in complex with a novel duplex intermediate of DNA annealing

This paper determines the structure of a bacteriophage RecT homolog bound to a novel DNA duplex intermediate, revealing insights into DNA annealing mechanisms.

Protein shape sampled by ion mobility mass spectrometry consistently improves protein structure prediction

This study shows that ion mobility mass spectrometry data improve protein structure prediction using Rosetta, achieving better accuracy for 15 out of 23 benchmark proteins.

HIV-1 Gag protein with or without p6 specifically dimerizes on the viral RNA packaging signal

This study shows that HIV-1 Gag and Gag{Delta}p6 specifically dimerize on Psi RNA, supporting a p6-independent mechanism for viral particle assembly initiation.