Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain.
M. Kowalski-Jahn, H. Schihada, A. Turku, T. Huber, T. P. Sakmar, G. Schulte
The paper develops BRET-based biosensors to study WNT-induced conformational changes in FZD CRD and shows CRD dynamics are not required for WNT/β-catenin signaling.
bioRxiv · May 1, 2021Cell Biology