Lysosome transporter purification and reconstitution identifies Ypq1 as a pH-gated lysine transporter
F. M. Arines, A. Wielenga, O. E. Burata, F. N. Garcia, R. B. Stockbridge, M. Li
The paper develops a method to overexpress, purify, and reconstitute the lysine transporter Ypq1 in yeast vacuoles, demonstrating its pH-gated transport function and lysine-dependent stability.
bioRxiv · Mar 31, 2023Biochemistry