Efficient, selectable marker free gene targeting in soybean using novel Ochrobactrum haywardense-mediated delivery
S. Kumar, Z.-B. Lui, N. Sanyour-Doyel, B. Landerts, A. Worden, A. Anand, H.-J. Cho, J. Bolar, C. Harris, L. Huang, A. Xing, A. Richardson
This paper develops a selectable marker-free gene targeting system in soybean using Ochrobactrum haywardense-mediated delivery, achieving 3.4% targeted insertion and 90% mutation rate in T0 plants.
bioRxiv · Oct 28, 2021 · PreprintPlant Biology