Engineering of highly active and diverse nuclease enzymes by combining machine learning and ultra-high-throughput screening
Neil Thomas, David Belanger, Chenling A. Xu, Hanson Lee, Kathleen Hirano, Kosuke Iwai, Vanja Polic, Kendra Dee Nyberg, Kevin G. Hoff, Lucas Frenz, Charlie A. Emrich, Jun W. Kim, Mariya Chavarha, Abi Ramanan, Jeremy J. Agresti, Lucy J. Colwell
This study combines machine learning and high-throughput screening to design improved nuclease enzymes, finding a more effective variant than standard directed evolution methods.
Cell Systems · Mar 1, 2025Peer-reviewedBioinformatics