Mariya Chavarha

Engineering of highly active and diverse nuclease enzymes by combining machine learning and ultra-high-throughput screening

This study combines machine learning and high-throughput screening to design improved nuclease enzymes, finding a more effective variant than standard directed evolution methods.

Functional architecture of intracellular oscillations in hippocampal dendrites

The paper uses two-photon imaging and electrophysiological recordings to study dendritic membrane potential dynamics in mouse hippocampal pyramidal cells during behavior, revealing spatial and temporal patterns of electrical signaling.

A positively tuned voltage indicator reveals electrical correlates of calcium activity in the brain

This paper introduces positively tuned GEVIs that enhance spike detection in vivo with standard imaging systems, offering higher temporal resolution than calcium indicators.

Imaging sensory transmission and neuronal plasticity in primary sensory neurons with genetically-encoded voltage indicator, ASAP4.4-Kv

This paper uses a novel genetically-encoded voltage indicator to study voltage dynamics in primary sensory neurons during various stimuli and injury, revealing synchronization and coding changes.

Two-photon voltage imaging of spontaneous activity from multiple neurons reveals network activity in brain tissue

This study uses two-photon voltage imaging to record electrical activity from multiple neurons in brain slices, revealing network connectivity with single-trial resolution.

Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

This paper demonstrates ultrafast two-photon fluorescence microscopy for in vivo neural activity imaging at high spatiotemporal resolution in awake mice.

Fast two-photon volumetric imaging of an improved voltage indicator reveals electrical activity in deeply located neurons in the awake brain

This paper introduces an improved voltage indicator and imaging method to enable high-speed, high-resolution voltage imaging in deeply located neurons of awake mice.

A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality

The paper introduces a new set of transgenic mouse lines for precise targeting and manipulation of brain cell types, enhancing genetic tools for neuroscience research.