A total synthetic approach to CRISPR/Cas9 genome editing and homology directed repair
Sara E. DiNapoli, Raúl Martinez-McFaline, Caitlin K. Gribbin, Paul J. Wrighton, Courtney A. Balgobin, Isabel S. Nelson, Abigail Leonard, Carolyn R. Maskin, Arkadi Shwartz, Eleanor D. Quenzer, Darya Mailhiot, Clara Kao, Sean C. McConnell, Jill L. O. de Jong, Wolfram Goessling, Yariv J. Houvras
The paper demonstrates the effectiveness of synthetic sgRNAs and dsDNA templates for efficient HDR and genome editing in zebrafish.
Nucleic Acids Research · Feb 5, 2020Peer-reviewedMolecular Biology