Rapid Production and Cryo-EM Structure Determination of GPCR-Nanodisc Complexes via Cell-Free Synthesis
Thomas A. Guilliam, Agata K Jakimowicz, Marco M. Manni, Zachary B. Gaber, Giulia Paris, Steven W. Hardwick, Lee R. Cooper, Dimitri Y. Chirgadze, Tobias WB Ost
This paper presents a cell-free method to rapidly produce and determine cryo-EM structures of GPCR-nanodisc complexes, achieving structural-grade results in four days.
bioRxiv · Oct 23, 2026 · PreprintBiochemistry