Development and characterisation of an optimised in vitro differentiation protocol for deriving hepatocyte-like cells from mouse embryonic stem cells
Beatrice Villani, Silviya Dimova, Abdulaziz Alhussini, Andrea Caporali, Congyue Chen, Ailsa Laird, C. Roland Wolf, Alistair P. D. Elfick, Richard R. Meehan, Sari Pennings
This study develops and evaluates two cytokine-based protocols to generate functional hepatocyte-like cells from mouse embryonic stem cells, with the advanced cocktail producing more mature cells.
bioRxiv · May 15, 2026 · PreprintCell Biology