A low-cost rpoB-based multiplex MAMA PCR for differentiation of the Klebsiella pneumoniae species complex
Mehbooba Sharmin, Al Amin, Hafizur Rahman, Nicol Janecko, Samir Kumar Saha, Yogesh Hooda, Arif Mohammad Tanmoy, Senjuti Saha
This paper develops a low-cost MAMA PCR assay for accurately differentiating members of the Klebsiella pneumoniae species complex using rpoB SNPs, achieving 100% sensitivity and specificity compared to WGS.
bioRxiv · Apr 15, 2026 · PreprintMicrobiology