High-efficiency HDR in immortalized cell lines by crude rAAV mediated donor template delivery
Stuti Mehta, Altantsetseg Buyanbat, Ge Zheng, Nan Liu, Stuart H. Orkin
This study demonstrates high-efficiency HDR-mediated knock-in of large DNA fragments in human and mouse cell lines using crude rAAV without purification.
bioRxiv · May 2, 2022 · PreprintMolecular Biology