Interstrand Crosslinks in Donor DNA Boost Gene Editing in Human Cells
Hannah I. Ghasemi, Julien Bacal, Amanda J. Yoon, Carmen Cruz, Jonathan T. Vu, Brooke M. Gardner, Chris D. Richardson
The paper shows that interstrand crosslinked DNA templates (xHDRTs) significantly boost gene editing efficiency in human cells by up to five-fold, independent of template topology or cell type.
bioRxiv · Mar 11, 2022 · PreprintMolecular Biology