Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci.
Guillaume Bernas, Mariette Ouellet, Andréa Barrios, Hélène Jamann, Catherine Larochelle, Emile H. Levy, Jean-François Schmouth
The paper describes a modified electroporation method to generate conditional allele mouse models using two strategies, achieving targeting efficiencies between 3-25% for eight genes.
BMC Biotechnology · May 12, 2022Peer-reviewedMolecular Biology