Comprehensive mutational scanning of a kinase in vivo reveals substrate-dependent fitness landscapes
Alexandre Melnikov, Peter Rogov, Li Wang, Andreas Gnirke, Tarjei S Mikkelsen
This study uses deep mutational scanning to show that fitness landscapes of a kinase depend on antibiotic structure and concentration, enabling the design of variants with orthogonal substrate specificities.
bioRxiv · Jun 10, 2014Molecular Biology