Development of a High-throughput in vivo Assay for the Determination of Adenylation Domain Specificities
Leonard Präve, Jing Liu, Yuanyuan Zhou, O. Nicolas Lonono Sanchez, Anna B. Wacker, Helge B. Bode
The paper develops an in vivo assay called ASCR to determine adenylation domain specificities in NRPS, testing 54 A domains from various organisms and confirming novel cyclic pentapeptides.
bioRxiv · Jul 15, 2026 · PreprintBiochemistry