Leonard Präve

The paper develops an in vivo assay called ASCR to determine adenylation domain specificities in NRPS, testing 54 A domains from various organisms and confirming novel cyclic pentapeptides.

This paper describes a Golden Gate assembly method for high-throughput engineering of non-ribosomal peptide synthetases (NRPS) to generate novel peptides and modify natural products.

The paper describes an evolution-inspired method to engineer NRPS by identifying a recombination breakpoint and using it to assemble NRPS fragments for producing a proteasome inhibitor.