Using double cut in vitro assembled CRISPR/Cas9 to modify the genome of Coccidioides posadasii
Heather Mead, Daniel R. Kollath, Ashley N. Itogawa, Austin V. Blackmon, Matthew M. Morales, Mitchell L. Bryant, Marcus de Melo Teixeira, Bridget M. Barker
The paper identifies a defensin-like gene in Coccidioides posadasii with high transcript levels in spherules and uses CRISPR-Cas9 to delete it.
bioRxiv · Mar 4, 2023 · PreprintMolecular Biology