Circularization of Single-Stranded DNA Donor Template Unleashes the Power of Non-Viral Gene Delivery for Long-Term HSCs editing
Gil Letort, Aymeric Duclert, Diane Le Clerre, Isabelle Chion-Sotinel, Roger Salvatori, Emilie Dessez, Margaux Sevin, Marco C. Rotondi, Cosimo Ducani, Philippe N. Duchateau, Julien Valton
This study develops a non-viral gene editing method using circular single-stranded DNA templates and TALEN, showing improved engraftment and long-term gene editing in HSPCs compared to AAV.
bioRxiv · Feb 19, 2025 · PreprintBioengineering