Systematic engineering of plant cytochrome P450 system identifies a comprehensive strategy for expression of highly functional P450 enzymes in Escherichia coli
M. Poborsky, C. Crocoll, M. S. Motawia, B. A. Halkier
This study systematically engineers plant cytochrome P450s to enhance their expression and activity in E. coli, identifying transmembrane domain truncation as a universal improvement strategy.
bioRxiv · Dec 13, 2022Bioengineering