Quantifying the spatiotemporal dynamics of IRES versus Cap translation with single-molecule resolution in living cells
A. L. Koch, L. Aguilera, T. Morisaki, T. J. Stasevich, B. E. Munsky
The paper develops a bicistronic biosensor to quantify IRES and Cap-dependent translation dynamics in living cells, revealing distinct kinetic behaviors and stress-induced shifts in translation efficiency.
bioRxiv · Jan 9, 2020Biochemistry