T. J. Stasevich

Single molecule imaging of the central dogma reveals myosin-2A gene expression is regulated by contextual translational buffering

The paper uses CRISPR-engineered cells to image MYH9 gene expression dynamics and shows translational buffering adjusts MYH9 protein levels in response to different cellular signals.

Generation and diversification of recombinant monoclonal antibodies for studying mitosis

This study develops methods to generate low-cost, high-yield recombinant monoclonal antibodies and antibody fragments for studying mitosis and other applications.

Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells

The paper introduces a method to visualize two endogenous chromatin loci in living cells using synthetic zinc-finger proteins and frankenbodies, revealing their dynamic association and separation upon cohesin disruption.

Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster

The paper demonstrates that single copies of short peptide tags can enable protein binding and manipulation in cultured cells and Drosophila, allowing degradation and relocalization of proteins of interest in vivo.

Quantifying the spatiotemporal dynamics of IRES versus Cap translation with single-molecule resolution in living cells

The paper develops a bicistronic biosensor to quantify IRES and Cap-dependent translation dynamics in living cells, revealing distinct kinetic behaviors and stress-induced shifts in translation efficiency.

Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag

The paper introduces Riboglow, a fluorescent RNA tag for detecting and tracking single mRNA molecules in live cells, and benchmarks it against the MS2 system.

Live-cell single RNA imaging reveals bursts of translational frameshifting

The paper uses live-cell single RNA imaging to study translational frameshifting in HIV-1 RNA, finding that ~8% of translating RNA robustly frameshift in living cells.

A genetically encoded probe for imaging HA-tagged protein translation, localization, and dynamics in living cells and animals

A genetically encoded HA frankenbody probe binds HA epitopes in living cells to track protein translation, localization, and dynamics in multiple contexts.