Accounting for RNA polymerase heterogeneity reveals state switching and two distinct long-lived backtrack states escaping through cleavage
R. Janissen, B. Eslami Mossallam, I. Artsimovitch, M. Depken, N. H. Dekker
The paper uses high-throughput magnetic tweezers to study E. coli RNA polymerase dynamics, identifying two long-lived backtrack pause states and showing RNA cleavage governs recovery from deep backtracks.
bioRxiv · Jan 6, 2021Biophysics