R. Janissen

Cohesin supercoils DNA during loop extrusion

This study shows cohesin supercoils DNA during loop extrusion, influencing genome architecture through both looping and supercoiling.

All eukaryotic SMC proteins induce a twist of -0.6 at each DNA-loop-extrusion step

The paper measures the twist induced by eukaryotic SMC proteins during DNA loop extrusion and finds all three SMC complexes induce a consistent -0.6 twist per step.

Single-molecule visualization of twin-supercoiled domains generated during transcription

This study visualizes twin-supercoiled domains formed during transcription using single-molecule experiments, showing equal upstream and downstream supercoiling without requiring RNA transcript drag.

Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation

This study investigates how ParB-DNA interactions form a dynamic partition condensate for bacterial chromosome segregation using in vitro experiments and simulations.

CTCF is a DNA-tension-dependent barrier to cohesin-mediated DNA loop extrusion

The paper shows CTCF blocks cohesin-mediated DNA loop extrusion and regulates its direction and loop size, revealing it as an active regulator rather than a passive barrier.

Controlled spatial organization of bacterial clusters reveals cell filamentation is vital for Xylella fastidiosa biofilm formation

This study shows that controlled spatial organization of Xylella fastidiosa cells reveals filamentation is essential for biofilm formation, driven by quorum sensing and cluster density.

Accounting for RNA polymerase heterogeneity reveals state switching and two distinct long-lived backtrack states escaping through cleavage

The paper uses high-throughput magnetic tweezers to study E. coli RNA polymerase dynamics, identifying two long-lived backtrack pause states and showing RNA cleavage governs recovery from deep backtracks.

Condensin extrudes DNA loops in steps up to hundreds of base pairs that are generated by ATP binding events

This study shows that condensin extrudes DNA loops in steps of up to 500 bp, driven by ATP binding, using magnetic tweezers and simulations.

Induced copy-back RNA synthesis as a novel therapeutic mechanism against RNA viruses

This study shows that a ribonucleotide analogue induces copy-back RNA synthesis, a new mechanism to combat RNA viruses by promoting defective viral genomes.