Successive remodeling of IgG glycans using a solid-phase enzymatic platform
B. F. Mann, Y.-P. Hsu, D. Verma, S. Sun, C. McGregor, I. Mangion
This study evaluates over 30 enzymes for glycoengineering immobilized IgG, demonstrating a solid-phase platform for efficient glycan remodeling and harmonization into complex-type N-glycoforms with high yield and retained binding affinity.
bioRxiv · Aug 27, 2021Biochemistry