Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries
J. Zhou, S. Li, K. K. Leung, B. K. ODonovan, J. Zou, J. DeRisi, J. A. Wells
This paper introduces SPD-NGS, a method using phage display libraries to deeply profile protease substrate specificity, identifying cleavage sites and natural substrates with high coverage and accuracy.
bioRxiv · May 9, 2020Biochemistry