K. K. Leung

Challenges in the discovery of tumor-specific alternative splicing-derived cell-surface antigens in glioma

The paper identifies tumor-specific alternative splicing events in glioma that could generate cell-surface antigens but finds significant spatiotemporal heterogeneity and lack of proteomic evidence.

Site-specific proximity labeling at single residue resolution for identification of protein partners in vitro and on cells

This paper optimizes a high-resolution proximity labeling method to identify protein partners of HER2 on cells and in vitro, finding 20 high-confidence neighbors enriched compared to non-specific labeling.

Computational pipeline provides mechanistic understanding of Omicron variant of concern neutralizing engineered ACE2 receptor traps

The paper develops a computational pipeline combining Rosetta and cryo-EM to analyze ACE2 receptor traps' binding to SARS-CoV-2 variants, showing high affinity and neutralization capabilities against Omicron and Delta.

Precision engineering of an anti-HLA-A2 chimeric antigen receptor in regulatory T cells for transplant immune tolerance

This paper develops an anti-HLA-A2 CAR for Tregs to induce transplant tolerance, showing they target HLA-A2-expressing grafts and suppress immune rejection.

CUB domain containing protein 1 (CDPC1) is a target for radioligand therapy in castration resistant prostate cancer

This paper evaluates CDCP1 as a target for radioligand therapy in mCRPC, showing its expression in most biopsies and demonstrating antitumor effects with 177Lu-4A06.

Targeting a proteolytic neo-epitope of CUB-domain containing protein 1 in RAS-driven cancer

The paper develops antibodies targeting proteolytic neo-epitopes of CDCP1 in RAS-driven cancers, showing improved tumor specificity and safety.

Defining the cell surface proteomic landscape of multiple myeloma reveals immunotherapeutic strategies and biomarkers of drug resistance

This study defines the myeloma cell surface proteome, identifies therapeutic targets and resistance biomarkers, and presents a miniaturized surface proteomics protocol.

Bi-paratopic and multivalent human VH domains neutralize SARS-CoV-2 by targeting distinct epitopes within the ACE2 binding interface of Spike

This paper identifies and engineers multivalent human VH domains that neutralize SARS-CoV-2 by targeting distinct epitopes on the Spike protein's ACE2 binding interface, showing increased affinity and potency.

Engineered ACE2 receptor traps potently neutralize SARS-CoV-2

This paper describes engineered ACE2 receptor traps that potently neutralize SARS-CoV-2 by blocking viral entry through enhanced binding affinity.

Trimeric SARS-CoV-2 Spike interacts with dimeric ACE2 with limited intra-Spike avidity

This study investigates the interaction between SARS-CoV-2 Spike and ACE2, showing that dimeric ACE2 binds with high affinity to Spike and blocks viral entry, with limited intra-Spike avidity.

Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries

This paper introduces SPD-NGS, a method using phage display libraries to deeply profile protease substrate specificity, identifying cleavage sites and natural substrates with high coverage and accuracy.

Broad and thematic remodeling of the surface glycoproteome on isogenic cells transformed with driving proliferative oncogenes

This study uses proteomics to show how six oncogenes remodel the surface glycoproteome of breast cells, with common functional themes reversed by MEK inhibition.