The graphene-based affinity cryo-EM grid for the endogenous protein structure determination
Sojin An, Eungjin Ahn, Tyler Koo, Soyoung Park, Boeon Suh, Krishna P. Rengasamy, Gaocong Lyu, Cheal Kim, Byung-Chul Kim, Hanseong Kim, Sang‐Ho Park, Dongyan Tan, Uhn‐Soo Cho
This paper introduces a graphene-based affinity cryo-EM grid (GFD-A) for capturing endogenous protein complexes, achieving resolutions of 2.71 Å for tagged proteins and 3.0–3.9 Å for endogenous RNase MRP and RNase P.
bioRxiv · Feb 22, 2025 · PreprintBiochemistry