High-resolution mapping of RNA structural maturation during Cas9 assembly with ABEL-FRET
Bok-Eum Choi, Hugh Wilson, Quan Wang
This study uses ABEL-FRET to map RNA structural changes during Cas9 RNP assembly, revealing how guide RNA flexibility is modulated through different assembly states.
bioRxiv · Sep 26, 2026 · PreprintBiophysics