Scaled and Efficient Derivation of Loss of Function Alleles in Risk Genes for Neurodevelopmental and Psychiatric Disorders in Human iPSC
Hanwen Zhang, Ada McCarroll, Lilia Peyton, Sol Díaz de León‐Guerrero, Siwei Zhang, Prarthana Gowda, David Sirkin, Mahmoud ElAchwah, Alexandra C. Duhe, Whitney Wood, Brandon Y Jamison, Gregory Tracy, Rebecca M. Pollak, Ronald P. Hart, Carlos N. Pato, , Jubao Duan
The paper develops a pipeline for efficient loss-of-function mutagenesis in NPD genes using base editing in hiPSCs, achieving high reproducibility and confirming neural phenotypes.
Stem Cell Reports · Sep 12, 2024Peer-reviewedGenetics