Relief of allosteric inhibition, redox imbalance, and transport limitations enables high-yield L-malate production in Escherichia coli
Moses Onyeabor, Lizbeth M. Nieves, Gavin Kurgan, Junpei Xiao, Logan Kurgan, Brittany Retallack, Haiwei Gu, Xuan Wang
This study improves an Escherichia coli strain for high-yield L-malate production by modifying allosteric regulation, redox balance, and transport, achieving a mass yield of 1.2 g malate per gram of glucose.
bioRxiv · May 7, 2026 · PreprintBioengineering