C. Iacobucci

Twisting Urea- to Imide-Based Mass Spectrometry-Cleavable Cross-Linkers Enables Affinity Tagging

This paper introduces DSSI, a new class of MS-cleavable cross-linkers with affinity tags, and validates its use in structural biology.

Structural Assessment of the Full-Length Wild-Type Tumor Suppressor Protein p53 by Mass Spectrometry-Guided Computational Modeling

This study uses mass spectrometry and computational modeling to assess the structure of full-length wild-type p53, revealing compaction of the C-terminal region and supporting a model of DNA binding.

First direct observation of 'elongated' conformational states in α-synuclein upon liquid-liquid phase separation

This study observes elongated conformational states of α-synuclein during liquid-liquid phase separation using XL-MS and COMPASS, providing insights into early stages of Parkinson's Disease.

Structural Analysis of 70S Ribosomes by Cross-Linking/Mass Spectrometry Reveals Conformational Plasticity

This study uses XL-MS to analyze E. coli ribosome structures, revealing conformational plasticity and remodeling 2,057 residues with high accuracy.

Flexibility of intrinsically disordered degrons in AUX/IAA proteins reinforces auxin co-receptor assemblies

The paper investigates how flexible intrinsically disordered regions in AUX/IAA proteins modulate auxin receptor assemblies by positioning AUX/IAAs on TIR1 for ubiquitylation.

Cross-linking/Mass Spectrometry: A Community-Wide, Comparative Study Towards Establishing Best Practice Guidelines

This paper summarizes current XL-MS practices, compares strategies, and proposes common protocols to establish best practice guidelines for accurate and reproducible results.