Real-time TIRF imaging of single adiponectin vesicle exocytosis in 3T3-L1 adipocytes stably expressing mCherry fused to human adiponectin
M. M. Shrestha, S. Barg, C. S. Olofsson
This study uses TIRF microscopy to observe and quantify single adiponectin vesicle exocytosis in 3T3-L1 adipocytes expressing mCherry-adiponectin, showing that both cAMP and Ca2+ elevation trigger release.
bioRxiv · Sep 13, 2022 · PreprintCell Biology