Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer
Martina F. Scallan, Catherine Dempsey, John A. MacSharry, Isabelle O’Callaghan, Paula M. O’Connor, Conor P. Horgan, Edel Durack, Paul D. Cotter, Sarah P. Hudson, H.A. Moynihan, Brigid Lucey
The paper validates a 4M GITC/Triton X-100 lysis buffer for SARS-CoV-2 RNA extraction, showing comparable results to commercial reagents during the COVID-19 pandemic.
bioRxiv · Apr 5, 2020 · PreprintMolecular Biology