Bio-synthesis of 15N-Labeled G-Quadruplexes to Investigate the Structure and Interactions in the Cell Lysate using Nuclear Magnetic Resonance
Mengbing Zou, Fujia Tian, Wenwen Zheng, Mengzhen Jiang, Shengkai Zhang, Xiaoli Liu, Changxing Ma, Meiping Zhao, Liang Dai, Conggang Li, Shenlin Wang
This paper introduces a method to produce 15N-labeled ssDNA for NMR studies of G-quadruplexes in cell lysates, revealing distinct ligand-binding behaviors in different environments.
bioRxiv · Nov 3, 2025 · PreprintBiochemistry