BiochemistrybioRxiv
High-throughput biochemical profiling reveals Cas9 off-target binding and unbinding heterogeneity
Evan August Boyle, Johan Oscar Lennart Andreasson, Lauren Mallory Chircus…·William James Greenleaf
The paper measures Cas9 off-target binding and unbinding kinetics using a high-throughput sequencing assay.
DOI 10.1073/pnas.1700557114