High-throughput biochemical profiling reveals Cas9 off-target binding and unbinding heterogeneity
Evan August Boyle, Johan Oscar Lennart Andreasson, Lauren Mallory Chircus, Samuel Henry Sternberg, Michelle Jennifer Wu, Chantal Katrin Guegler, Jennifer Anne Doudna, William James Greenleaf
The paper measures Cas9 off-target binding and unbinding kinetics using a high-throughput sequencing assay.
bioRxiv · Jun 19, 2016Biochemistry