Molecular BiologybioRxivPreprint
Successful optimization of CRISPR/Cas9-mediated defined point mutation knock-in using allele-specific PCR assays in zebrafish
Sergey V. Prykhozhij, Charlotte J. Fuller, Shelby L. Steele…·Jason N. Berman
The paper optimizes CRISPR/Cas9-mediated point mutation knock-in in zebrafish using allele-specific PCR assays and identifies key factors improving knock-in efficiency and reducing false positives.
2 citations