Sergey V. Prykhozhij

This study characterizes zebrafish models of Li-Fraumeni syndrome with TP53 mutations, showing dysfunctional p53 pathways, tumor development, and mutation-specific phenotypes.

CRISPR Knock-in Designer automatically designs DNA oligonucleotides for precise point mutations using CRISPR/Cas9, supporting various experimental strategies and genomic contexts.

The paper optimizes CRISPR/Cas9-mediated point mutation knock-in in zebrafish using allele-specific PCR assays and identifies key factors improving knock-in efficiency and reducing false positives.